Stephen Lutz Circular Permutation

Circular permutation a different way to engineer enzyme structure and function Ying Yu and Stefan Lutz Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA, 30322 Protein engineers traditionally rely on amino acid sub-stitutions to alter the functional properties of biomacro-molecules, yet have largely overlooked the potential

Protein engineering by random circular permutation is an effective tool for tailoring protein topology with potential functional benefits including improved catalytic activity.

Ying Yu 1 , Stefan Lutz. Affiliation 1 Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, GA 30322, USA. PMID 21087800 yet have largely overlooked the potential benefits of reorganizing the polypeptide chain of a protein by circular permutation CP. By connecting the native protein termini via a covalent linker and

In addition to the well-known example of directed evolution - an iterative two-step process of diversification and selection modeled after Darwinian evolution 1, 2, 3 - a less-established method called circular permutation CP has recently been adapted by protein engineers as a new approach to diversify and tailor protein structure and

Circular permutation CP is an invaluable approach to redesigning a protein by rearranging the amino acid sequence, where the connectivity of the secondary structural elements is altered without changing the overall structure of the protein. Yu and Lutz examined the effects of different types and lengths of linkers used to link the

Yu, Y., amp Lutz, S. 2011. Circular permutation a different way to engineer enzyme structure and function. Trends in Biotechnology, 291, 18-25. doi10.1016j

The Structure of a Thermophilic Kinase Shapes Fitness upon Random Circular Permutation. ACS Synthetic Biology 2016, 5 5 , Mark Okon, Natalie C. J. Strynadka, Stefan Lutz, Stephen G. Withers and Lawrence P. McIntosh. Circular Permutation of Bacillus circulans Xylanase A Kinetic and Structural Study. Biochemistry 2010, 49 11 ,

Glenna E. Meister. Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218-2681, USA

Circular permutation can be the result of evolutionary events, post-translational modifications, or artificially engineered mutations. Yu Y, Lutz S. Circular permutation a different way to engineer enzyme structure and function. Trends Biotechnol. 20112918-25. doi 10.1016j.tibtech.2010.10.004.

Qian Z, Lutz S 2005 Improving the catalytic activity of Candida antarctica lipase B by circular permutation. J Am Chem Soc 12713466-13467. Article CAS PubMed Google Scholar Qian Z et al 2009 Structural redesign of lipase B from Candida antarctica by circular permutation and incremental truncation.